The mission of The Translational and Molecular Imaging Institute (TMII) is to research, develop, and apply a new generation of translational and molecular imaging methodologies. Once confined to clinical radiology and basic cell biology, imaging tools now can facilitate studies of gene expression at the sub-cellular level, helping scientists unveil the secrets of all living systems. Researchers and clinicians have comprehensive access to cutting edge imaging equipment, including magnetic resonance imaging (MRI), positron emission tomography (PET), computed tomography (CT), and emerging modalities such as bioluminescent and fluorescent imaging. At the Institute, we envision interdisciplinary collaboration and application of these technologies as keys to building on our research capabilities, increasing our understanding of disease processes, and improving diagnostic accuracy. TMII looks to be at the forefront of biomedical imaging, which in the 21st century will offer unparalleled opportunities to transform clinical medicine.